For Automated Extraction of Genomic DNA from Cultured Cells and Amniotic Fluids
MagCore® Cultured Cells DNA Kit can be used to extract DNA from up to 5 x 106 cells via MagCore® automated extraction instruments. Different protocols are optimized for samples of cell suspensions, cells grown in a monolayer, and amniotic fluid from trypsinization to DNA elution. Our programs and protocols ensure maximal efficacy and efficiency in performance for the specific type of sample used.
Product
Code 110 MagCore® Cultured Cells DNA Kit
Features
- Consistent and reproducible results.
- Cartridges are pre-filled and sealed to prevent contamination
- Minimal manual pipetting, complete automatic procedure from cell lysis to elution
Applications
MagCore® automated extraction systems allow processing multiple sample types in the same run, including:
- Cultured cell suspension
- Cells grown in a monolayer
- Amniotic fluid
Purified DNA available for direct usage in downstream applications, such as:
- PCR and real-time quantitative PCR
- Southern Blot
- Next Generation Sequencing
- Genotyping
Performance
Cells-1
Table 1. Consistency in yield and reproducibility of results shown in HeLa cell DNA extraction using MagCore® systems. HeLa cell cultures in 2-fold serial dilutions were subjected to DNA extraction. All samples were treated with RNase A to remove RNA contaminants. Duplicates of samples with different amounts of cells were analyzed: 4 x 106 cells, 2 x 106 cells, 1 x 106 cells, and 5 x 105 cells. The yield and purity from samples of 200µl cultured cell suspensions were measured by Nanodrop n-1000. Lane 1/2: 4 x 106 Hela cells, Lane 3/4: 2 x 106 Hela cells; Lane 5/6: 1 x 106 cells; Lane 7/8: 5 x 105 cells and Lane M: RBC 1kb ladder marker.
Cells-2
Figure 1. Consistency in yield and reproducibility of results shown in HeLa cell DNA extraction using MagCore® systems. HeLa cell cultures in 2-fold serial dilutions were subjected to DNA extraction. All samples were treated with RNase A to remove RNA contaminants. Duplicates of samples with different amounts of cells were analyzed: 4 x 106 cells, 2 x 106 cells, 1 x 106 cells, and 5 x 105 cells. The yield and purity from samples of 200µl cultured cell suspensions were measured by Nanodrop n-1000. Lane 1/2: 4 x 106 Hela cells, Lane 3/4: 2 x 106 Hela cells; Lane 5/6: 1 x 106 cells; Lane 7/8: 5 x 105 cells and Lane M: RBC 1kb ladder marker.
Cells-3
Table 2. Results of cell DNA extraction from amniotic fluid by Nanodrop n-1000. Cells were harvested from 10-15ml amniotic fluid from healthy females pregnant for 16-18 weeks. Purified by MagCore® instruments, the DNA yield varied between individuals due to different body conditions. However, the purity (A260/A280) were consistent in the range of 1.8 ±0.1.
Cells-4
Figure 2. Agarose gel electrophoresis results of cell DNA from amniotic fluid. Cells were harvested from 10-15ml amniotic fluid from healthy females pregnant for 16-18 weeks. Purified by MagCore® automated extraction, the concentration of cell DNA varied between individuals due to different body conditions. Lane 1: GPT2967P; Lane 2: GPT2952P; Lane 3: NP679P; Lane 4: NP777P; Lane 5: NP795P; and Lane M: RBC 1kb ladder marker.
Specifications
| MagCore® Plus II/Super/HF16 Plus | |||
|---|---|---|---|
| Cartridge Code | 36 Preps Cat No. | 96 Preps Cat No. | Running Time |
| 110 | MCC-01 | MCC-02 | 39 mins (sample volume: 200µl, up to 5 x 106 cells) |
| Components Available for Purchase | ||
|---|---|---|
| Product | Cat No. | Content |
| Proteinase K Set | PK011 | 11 mg Proteinase K, 1.25 ml PK Storage Buffer |
| RNase A | RN050 | 50µl RNase A (50mg/ml) |
| RN130 | 130µl RNase A (51mg/ml) | |