For Automated Extraction of Genomic DNA from Human Whole Blood samples
MagCore® Genomic DNA Whole Blood Kits are designed for extracting total DNA (including genomic, mitochondrial, and viral DNA), from samples containing whole blood, plasma, serum and buffy coat. With built-in programs for all extraction kits, run your blood DNA extraction on all MagCore® instruments by simply a few clicks. Purified DNA of approximately 20-30 kb is suitable for PCR or other downstream applications.
Product Series
Code 101 MagCore® Genomic DNA Whole Blood Kit (Speedy Installation)
Code 102 MagCore® Genomic DNA Whole Blood Kit
Code 104 MagCore® Genomic DNA Large Volume Whole Blood Kit
Code 106 MagCore® Genomic DNA Whole Blood Kit (For Genotyping)
Features
- Consistent and reproducible results.
- Cartridges are pre-filled and sealed to prevent contamination
- Minimal manual pipetting, complete automatic procedure from cell lysis to elution
Applications
MagCore® automated extraction systems allow processing multiple sample types in the same run, including:
- Fresh and frozen whole blood
- Buffy coat
- Body fluids
Purified DNA available for direct usage in downstream applications, such as:
- PCR and real-time quantitative PCR
- Southern Blot
- Next Generation Sequencing
- Genotyping
Performance
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Table 1. Performance of MagCore® Genomic DNA extraction on human whole blood samples treated with various anticoagulants. The performance of DNA extraction from human whole blood samples containing EDTA, sodium citrate, sodium fluoride and lithium heparin from different types of blood collection tubes. Concentration and purity of isolated genomic DNA are measured by Nanodrop n-1000.
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Figure 1. Analysis of Genomic DNA extracted from human whole blood samples using gel electrophoresis. Performance of MagCore® automated extraction analyzed on agarose gel electrophoresis; Lane M: RBC 1 kb DNA ladder Marker; Lane 1-5: Genomic DNA from human whole blood samples collected in tubes containing EDTA, sodium citrate, lithium heparin (4ml), sodium fluoride, and lithium heparin (9ml).
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Figure 2. Analysis of DNA product integrity on gene Cbl-b (1.7kb) from various human whole blood samples. Effect of anticoagulants on the integrity of DNA isolated from MagCore® instruments was investigated on agarose gel electrophoresis. Lane 1-5: PCR product of gene Cbl-b from human whole blood samples collected in tubes containing EDTA, sodium citrate, lithium heparin (4ml), sodium fluoride, and lithium heparin (9ml). Lane P: Positive Control; and Lane M: RBC 1 kb DNA ladder Marker.
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Figure 3. Linear relationship between DNA yield and different sample dilutions. Human whole blood samples of 20, 50, 80, 100, 150, and 200μl from a single donor were diluted to 200μl using PBS buffer. The amount of DNA was calculated using A260 results. No significant difference was observed in the DNA yield from eluates of 100μl and 200μl.
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Figure 4. Consistency in yield and purity isolated from human whole blood of different sample volumes. The quality of human whole blood DNA from 16 individuals was studied using Nanodrop n-1000. Yield and purity of DNA isolated from MagCore® instruments were calculated from A260 and A260 /A280. The average amount of DNA yielded from 400µl samples was similar to two times the amount of DNA from 200µl samples along with similar purity.
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Figure 4. Consistency in yield and purity isolated from human whole blood of different sample volumes. The quality of human whole blood DNA from 16 individuals was studied using Nanodrop n-1000. Yield and purity of DNA isolated from MagCore® instruments were calculated from A260 and A260 /A280. The average amount of DNA yielded from 400µl samples was similar to two times the amount of DNA from 200µl samples along with similar purity.
Specifications
| MagCore® Plus II/Super/HF16 Plus | |||
|---|---|---|---|
| Cartridge Code | 36 Preps Cat No. | 96 Preps Cat No. | Running Time |
| 101 | MGB400-01 | MGB400-02 | 39 mins (sample volume: 200µl) |
| 50 mins (sample volume: 400µl) | |||
| 102 | MVN400-03 | MVN400-04 | 39 mins (sample volume: 200µl) |
| 50 mins (sample volume: 400µl) | |||
| 104 | MGB1200 | 82 mins (sample volume: 1200µl) | |
| 106 | MGB400-07 | MGB400-08 | 41 mins (sample volume: 200µl) |
| 53 mins (sample volume: 400µl) | |||
| Optical Detection is not provided | |||
| MagCore® HF48 | ||||
|---|---|---|---|---|
| Cartridge Code | 36 Preps Cat No. | 96 Preps Cat No. | Running Time (24 samples) | Running Time (48 samples) |
| 101 | MGB400-01 | MGB400-02 | 47 mins (sample volume: 200µl) | 98 mins (sample volume: 200µl) |
| 58 mins (sample volume: 400µl) | 121 mins (sample volume: 400µl) | |||
| 102 | MGB400-03 | MGB400-04 | 47 mins (sample volume: 200µl) | 47 mins (sample volume: 200µl) |
| 58 mins (sample volume: 400µl) | 58 mins (sample volume: 400µl) | |||
| 104 | MGB1200 | 878 mins (sample volume: 1200µl) | 161 mins (sample volume: 1200µl) | |
| 106 | MGB400-07 | MGB400-08 | 47 mins (sample volume: 200µl) | 98 mins (sample volume: 200µl) |
| 58 mins (sample volume: 400µl) | 57 mins (sample volume: 400µl) | |||
| Components Available for Purchase | ||
|---|---|---|
| Product | Cat No. | Content |
| Proteinase K Set | PK011 | 11 mg Proteinase K, 1.25 ml PK Storage Buffer |
| RNase A | RN050 | 50µl RNase A (50mg/ml) |
| RN130 | 130µl RNase A (51mg/ml) | |