For Automated Extraction of Genomic DNA from Tissue, FFPE Tissue and Forensic Samples
MagCore®Genomic DNA Tissue Kit is designed for purification of total DNA (including genomic, mitochondrial and viral DNA) from a variety of animal tissues or cells by using MagCore® auto-extraction instrument. The provided Filter Column can filtrate hard tissue sample or swab sample to prevent tissue residues to obstruct pipette tip during the process of MagCore® . The method uses pre-filled cartridge contains proteinase K and a chaotropic salt to lysis cells and degrade protein. DNA will bind to cellulose coated magnetic beads . After washing off the contaminants, the purified DNA is eluted by low salt elution buffer. Purified DNA of approximately 20-30 kb in length is suitable for PCR or other enzymatic reactions.
Product
Code 401 MagCore® Genomic DNA Tissue Kit
Features
- Consistent and reproducible results
- Cartridges are pre-filled and sealed to prevent contamination
- Minimal manual pipetting, complete automatic procedure from cell lysis to elution
Application
MagCore® automated extraction systems allow processing multiple sample types in the same run, including:
- Animal Tissues: Solid animal tissue, Stool samples
- Formalin-fixed, Paraffin-embedded (FFPE) tissues samples
- Cultured Yeast samples
- Forensics samples: Dried blood spot, Saliva stain, Swab, Cigarette butts, Hair roots, Chewing gum, Animal feed, Soil and Betel nut residue
Purified DNA available for direct usage in downstream applications, such as:
- PCR and real-time quantitative PCR
- Southern Blot
- Next Generation Sequencing
- Genotyping
Performance
Specifications
| MagCore® Plus II/Super/HF16 Plus | |||
|---|---|---|---|
| Cartridge Code | 36 Preps | 72 Preps | Running Time |
| Cat No. | Cat No. | ||
| 401 | MGT-01 | MGT-03 | 33 mins (sample volume: 400µl) |
| *Optical detection not provided | |||
| Components Available for Purchase | ||
|---|---|---|
| Product | Cat No. | Content |
| Proteinase K Set | PK011 | 11 mg Proteinase K, 1.25 ml PK Storage Buffer |
| RNase A | RN050 | 50µl RNase A (50mg/ml) |
| RN130 | 130µl RNase A (51mg/ml) | |