For Automated Extraction of Genomic DNA from Tissue, FFPE Tissue and Forensic Samples

MagCore®Genomic DNA Tissue Kit is designed for purification of total DNA (including genomic, mitochondrial and viral DNA) from a variety of animal tissues or cells by using MagCore® auto-extraction instrument. The provided Filter Column can filtrate hard tissue sample or swab sample to prevent tissue residues to obstruct pipette tip during the process of MagCore® . The method uses pre-filled cartridge contains proteinase K and a chaotropic salt to lysis cells and degrade protein. DNA will bind to cellulose coated magnetic beads . After washing off the contaminants, the purified DNA is eluted by low salt elution buffer. Purified DNA of approximately 20-30 kb in length is suitable for PCR or other enzymatic reactions.

Product
Code 401 MagCore® Genomic DNA Tissue Kit
Features
  1. Consistent and reproducible results
  2. Cartridges are pre-filled and sealed to prevent contamination
  3. Minimal manual pipetting, complete automatic procedure from cell lysis to elution
Application

MagCore® automated extraction systems allow processing multiple sample types in the same run, including:

  • Animal Tissues: Solid animal tissue, Stool samples
  • Formalin-fixed, Paraffin-embedded (FFPE) tissues samples
  • Cultured Yeast samples
  • Forensics samples: Dried blood spot, Saliva stain, Swab, Cigarette butts, Hair roots, Chewing gum, Animal feed, Soil and Betel nut residue

 

Purified DNA available for direct usage in downstream applications, such as:

  1. PCR and real-time quantitative PCR
  2. Southern Blot
  3. Next Generation Sequencing
  4. Genotyping
 
Performance
  • 401-01

    Figure 1. Genomic DNA was purified from 10mg of mice tissue samples and 0.25cm tail.
  • 401-02

    Figure 2. Genomic DNA from 10mg of various animal tissues were extracted by the MagCore®401 Genomic DNA Tissue Kit. DNA was eluted in 60 µL and quantitated using Nanodrop n-1000.
  • 401-03

    Figure 3. Agarose gel electrophoresis of the various animal tissue DNA was isolated from MagCore® automated extraction. Lane M: RBC 1 kb DNA ladder Marker; Lane 1: Rat kidney; Lane 2: Rat liver; Lane 3-4: Pork liver; Lane 5-6: Fish tissue.
Specifications
MagCore® Plus II/Super/HF16 Plus
Cartridge Code36 Preps72 PrepsRunning Time
Cat No.Cat No.
401MGT-01MGT-0333 mins (sample volume: 400µl)
*Optical detection not provided

 

Components Available for Purchase
ProductCat No.Content
Proteinase K SetPK01111 mg Proteinase K, 1.25 ml PK Storage Buffer
RNase ARN05050µl RNase A (50mg/ml)
RN130130µl RNase A (51mg/ml)