For Automated Extraction of Genomic DNA from Formalin-fixed Paraffin-embedded (FFPE) Tissue
MagCore® Genomic DNA FFPE One-Step Kit is designed to purify total DNA (including genomic, mitochondrial, and viral DNA) from Formalin-fixed Paraffin-embedded tissues via MagCore® Automated Extraction instruments. Our program features One-Step Heating, which automatically melts paraffin and lyses cells at the same time. No harmful reagent such as xylene is involved in the deparaffination process. Two protocols are outlined for different tissues sizes: 2-hour setup for smaller tissues and 16-hour setup for larger tissues.
Product
Code 405 MagCore® Genomic DNA FFPE One-Step Kit
Features
- Can load FFPE tissue samples with a surface area of up to 300 mm2 and up to 5µm thick
- Minimal manual pipetting, complete automatic procedure from deparaffination to elution
- Stable deparaffination by Sula oil and reproducible results
- Cartridges are pre-filled and sealed to prevent contamination
Applications
MagCore® automated extraction systems allow processing multiple sample types in the same run, including:
- Formalin-fixed, Paraffin-embedded (FFPE) tissues samples
Purified DNA available for direct usage in downstream applications, such as:
- PCR and real-time quantitative PCR
- Southern Blot
- Next Generation Sequencing
- Genotyping
Performance
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Figure 1A. Comparison of DNA yield extracted from MagCore® Genomic DNA FFPE One-Step Kit and Competitor Q product. It is shown that the DNA extracted by MagCore® has a concentration higher on average than competitor Q in results obtained from Nanodrop N-1000. Smaller samples were subjected to 2 hours of heating and larger samples to 16 hours during the extraction to ensure thorough deparaffination.
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Figure 2. PCR results of DNA purified by MagCore® and Competitor Q on an 1.5% agarose gel electrophoresis. Overall, the PCR product yields are higher from DNA extracted using MagCore® than competitor Q in various sample sizes. Smaller samples were subjected to 2 hours of heating and larger samples to 16 hours during the extraction to ensure thorough deparaffination.
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Figure 3. Analysis of DNA yielded from 10 mg of various mouse tissue samples and 0.25 cm mouse tail by Nanodrop N-1000. Tissue DNA was extracted by MagCore® Genomic DNA FFPE One-Step Kit. Different amounts of DNA were extracted from different tissues for different cell density, genetic expression levels and cell replication frequency.
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Figure 4A. Real Time PCR results of DNA purified by MagCore® and Competitor Q. The Ct values of PCR product from MagCore® extracted DNA are mostly less or equal to those of Competitor Q. The analysis indicates that DNA can be isolated with a higher yield from MagCore® products. Smaller samples were subjected to 2 hours of heating.
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Figure 4B. Real Time PCR results of DNA purified by MagCore® and Competitor Q. The Ct values of PCR product from MagCore® extracted DNA are mostly less or equal to those of Competitor Q. The analysis indicates that DNA can be isolated with a higher yield from MagCore® products. Smaller samples were subjected to 2 hours of heating.
Specifications
| MagCore® Plus II/Super/HF16 Plus | |||
|---|---|---|---|
| Cartridge Code | 36 Preps | 72 Preps | Running Time |
| Cat No. | Cat No. | ||
| 405 | MGF-01 | MGF-03 | 162 mins (2-hour Heating - Standard) |
| 989 mins (16-hour Heating - High Yield) | |||
| Components Available for Purchase | ||
|---|---|---|
| Product | Cat No. | Content |
| Proteinase K Set | PK011 | 11 mg Proteinase K, 1.25 ml PK Storage Buffer |
| RNase A | RN050 | 50µl RNase A (50mg/ml) |
| RN130 | 130µl RNase A (51mg/ml) | |